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Banik, A. K.
- Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200
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Authors
Affiliations
1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
Source
Asian Journal of Research in Chemistry, Vol 6, No 7 (2013), Pagination: 613-617Abstract
Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.Keywords
Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, Mutant
References
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- Ganguly S., Banik A.K., Production of L-glutamic acid by Immobilized Micrococcus glutamicus AB100 Asian J. Research Chem. 4(6): June, 2011.
- Gupta A, Das S.K and Banik A.K, Induced mutation and selection of a high yielding strain of Micrococcus glutamicus for L-lysine production. International Journal of Pharma and Bio Science. 3(2): 2012; B35-B42.
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- Ganguly S. and Banik A.K. Production of L-glutamic acid by Immobilized Micrococcus Glutamicus AB100. Asian journal of research in chemistry.
- Shah A.H., Hameed A, Ahmad S. and Khan GM. Optimization of culture condition for L-lysine fermentation by Corynebacterium glutamicum. Online Journal of Biological Science. 2(3); 2002: 151-156
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- Development of a Mutant Strain of Thiobacillus ferrooxidans and Optimisation of some Physical Parameters for Desulfurization of Indian Coal
Abstract Views :184 |
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Authors
Affiliations
1 Department of Chemical Engineering University of Calcutta, 92, A. P. C. Road, Calcutta - 700 009, IN
1 Department of Chemical Engineering University of Calcutta, 92, A. P. C. Road, Calcutta - 700 009, IN
Source
Journal of Surface Science and Technology, Vol 15, No 1-2 (1999), Pagination: 30-40Abstract
A mutant strain of Thiobacillus ferrooxidans X200 was incubated with samples of coal taken from Assam, India. Mutation resulted in a greater amount of desulfurization (30-32%) against the parent strain (9.9-14%). Influence of leaching parameters such as ore concentration (pulp density), particle size, initial pH of the medium, temperature, volume of medium in 250 ml conical flask, inoculum concentration and age of the inoculum was studied. Optimum particle size was 150 to 200 mesh, optimum pulp density was 2%, initial pH of the medium was 6.0, optimum volume of fermentation medium was 50 ml, time period of incubation was 8 days, inoculum volume was 5.0 ml and age of inoculum, 7 days. The maximum desulfurization of coal by using these physical parameters was 55%.Keywords
Thiobacillus ferrooxidans, X200, Coal, Desulfurization.- Nutritional Effect on Acid Phosphatase Activity during the Solubilisation of Indian Rock Phosphate by Aspergillus niger
Abstract Views :135 |
PDF Views:1
Authors
Runa Ghosh
1,
A. K. Banik
1
Affiliations
1 Department of Chemical Engineering, University of Calcutta, 92, Acharya Prafulla Chandra Road, Calcutta-700 009, IN
1 Department of Chemical Engineering, University of Calcutta, 92, Acharya Prafulla Chandra Road, Calcutta-700 009, IN
Source
Journal of Surface Science and Technology, Vol 14, No 1-4 (1998), Pagination: 286-298Abstract
Aspergillus niger treated with ethyl methane sulfonate and gamma rays was incubated with samples of rock phosphate taken from Rajasthan, India for exploring its solubilisation capacity and phosphatase activity. 4 percent glucose and 0.4 percent sodium nitrate were the best carbon and nitrogen sources respectively. 0.1 percent KCl and 0.03 percent KH2PO4, stimulated the solubilisation process whereas CaCO3 inhibited it. Phosphatase activity was found to vary directly with the solubilisation process indicating that the dephosphorylation of the rock was due to the production of acid phosphatase enzyme.Keywords
Aspergillus niger, Rock Phosphate, Solubilisation, Phosphatase Activity.- Production of L-Glutamic Acid by Immobilized Micrococcus glutamicus Ab100
Abstract Views :162 |
PDF Views:0
Authors
S. Ganguly
1,
A. K. Banik
1
Affiliations
1 Department of Chemical Engineering, Bio-chemical Engineering Division, Bio-Technology Laboratory, University of Calcutta, Kolkata – 700009, West Bengal, IN
1 Department of Chemical Engineering, Bio-chemical Engineering Division, Bio-Technology Laboratory, University of Calcutta, Kolkata – 700009, West Bengal, IN